Babesia gibsoni and Babesia canis are the etiological agents of canine babesiosis, a protozoal hemolytic disease with global significance. Canine babesiosis has been diagnosed by microscopic identification of intra-erythrocytic trophozoites in blood smear, and by serological testing. Here we developed a quantitative fluorescence resonance energy transfer (FRET)-PCR that amplifies a fragment of ...

Canine babesiosis caused by Babesia gibsoni was diagnosed in a 3-month-old Pit-bull pup during a routine clinical examination. Diagnosis was confirmed by way of smear examination, PCR, Reverse Line Blot (RLB) and sequence analysis which showed 100% homology with B. gibsoni (Japan AB118032) and Babesia sp. (Oklahoma) (AF205636). Haematology showed moderate anaemia and severe thrombocytopenia. Tr...

| An epidemiological study of babesiosis in stray dogs was conducted at Chittagong Metropolitan area, Bangladesh, for six months. Blood samples from 130 stray dogs were collected from nine randomly selected areas along with the information of age and sex. Blood samples were initially examined by Giemsa’s stained blood smear method and DNA was extracted and Babesia spp. were confirmed by amplify...

A nationwide survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan was conducted using molecular methods. A total of 1,341 H. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by B. gibsoni-targeted PCR. Partial sequence analysis revealed that 12 of 13 positive samples sequenced, inclu...

In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confi...

The objective of this study was to determine whether different isolates of Babesia microti could be distinguished from morphologically similar isolates of B. gibsoni by using a ribosomal DNA (rDNA) probe. A Babesia-specific rDNA probe was obtained by polymerase chain reaction amplification of sequences from B. microti DNA using universal primers directed against highly conserved portions of the...

Aim: To determine the seroprevalence of vector(tick)-borne Babesia canis, Babesia gibsoni, Ehrlichia canis and Borrelia burgdorferi and mosquito-borne Dirofilaria immitis in naturally exposed pet, working, and street dogs in Assam, India. Materials and Methods: Seroprevalence of B. canis and B. gibsoni was determined using Indirect Fluorescent Antibody Test (IFAT) kit and for D. immitis, E. can...

BACKGROUND Dogs seized by law enforcement agencies during dogfighting investigations are at increased risk of Babesia gibsoni infection. A rapid and cost-effective diagnostic test would increase the feasibility of mass screening of dogs for infection and monitoring treatment efficacy in B. gibsoni-infected dogs. OBJECTIVE To determine the performance of a point-of-need insulated isothermal PC...

A retrospective and prospective analysis of clinical records of dogs diagnosed with Babesia infections was carried out for the years 2000 to 2013 from practices in Lusaka, Zambia. Records of 363 dogs with confirmed Babesia infections were analysed using demographic factors including sex, breed, age, and clinical signs in relation to haematological findings and Babesia species. The clinical and ...

Babesia canis and B. gibsoni are responsible for canine babesiosis throughout the world. Babesia canis consists of a group of three biologically different subspecies, namely B. c. canis, B. c. vogeli, and B. c. rossi. Babesia gibsoni is subdivided into 2 subspecies: the North American and Asian subspecies. Babesia c. canis is found in Europe, B. c. vogeli in northern Africa, North America and S...